中试及生产型微生物发酵设备

Tank Body

Design Pressure: ≤0.3 MPa, Working Pressure: ≤0.15 MPa. Features an optimized flow guidance design. Internal and external surface polish with a precision of Ra ≤ 0.4 µm. The tank is equipped with: a manhole, sight glass, ports for pH, DO, and temperature electrodes. The tank lid includes a flame inoculation port and various process ports.

Agitation

Top-mounted mechanical agitation system with an aseptic sealing system. The agitation structure allows for easy disassembly and features an adjustable shaft with a mechanical seal assembly. Agitator Blades: Multi-configuration combined agitator blades, including a 1-stage high-efficiency comb-type defoaming blade (standard).

Air Inlet System

Air Inlet Method: Deep sparging from the tank bottom (optional ring sparger available). Air Sterilization Filtration: Equipped with a pre-filtration system (0.3 µm) and a sterilizing-grade filtration system (0.01 µm).

Piping System

Piping installation complies with microbial fermentation requirements, designed to be dead-leg free and prevent material residue. All wetted parts, including pipes and valves, utilize sanitary-grade diaphragm valves and polished inner surface pipes.

Equipment

Control Functions

Temperature

pH

DO

Rotation Speed

Flow Rate

Pressure

Sterilization

Feeding

Seed Tank

Monitor & Control

Monitor & Control

Monitor & Control

 Variable Frequency

Auto / Manual

Auto / Manual

Auto / Manual

Peristaltic Pump / Auto Diaphragm Valve

Fermenter

Monitor & Control

Monitor & Control

Monitor & Control

 Variable Frequency

Auto / Manual

Auto / Manual

Auto / Manual

Peristaltic Pump / Auto Diaphragm Valve

Temperature

Four-digit display temperature transmitter with a matching PT100 temperature electrode.
Measurement Accuracy: Class 0.3 (0.3% FS ± 0.1°C).
Measurement Range: 0-150°C.
Control Method: Digital setting, manual/automatic control.
Actuator: Pneumatic valve / hot water circulation pump.
Control Content: Bidirectional control for heating and cooling.

pH

World-renowned Swiss brand pH glass gel electrode.
Electrode allows for in-situ high-temperature sterilization (100°C-130°C).
Equipped with a stainless steel pH electrode sheath.
Measurement Range: 0-14, Accuracy: ±0.01.
Four-digit display transmitter, power supply AC 220V, output 4-20mA.
Fitted with original Swiss imported electrode shielded cable.

DO

World-renowned Swiss brand DO membrane electrode.
Electrode allows for in-situ high-temperature sterilization (100°C-130°C).
Equipped with a stainless steel DO electrode sheath.
Measurement Range: 0-100%, Accuracy: ±1%.
Matching four-digit display transmitter, power supply AC 220V, output 4-20mA.

Rotation Speed

Variable-frequency drive (VFD) stepless speed regulation system, power-matched with the motor.
Digital setting of rotation speed value.
Actuator: Frequency converter.

Sterilization

Digital setting of sterilization temperature and time. Automatic control of sterilization temperature and cooling process.
Sterilization Temperature Range: 80°C-125°C.
Sterilization Process: Steam heats the jacket to pre-warm the tank. After reaching preheat temperature, direct steam injection into the tank (optional setting) can be selected to achieve sterilization temperature.
Automatic temperature control begins upon start of timer.
After sterilization time elapses, automatic cooling to the set fermentation temperature occurs.

Pressure

Digital setting of internal tank pressure, with linear regulation via 4-20mA signal.

Flow Rate (Optional)

Digital setting of inlet gas flow rate, automatically controlled by a proportional regulating valve.

Foam Control

Control Method: Manual/Automatic control.
Control Content: Digital setting with delayed quantitative control.
Actuator: Includes foam alarm function.

Feeding

Control Method: Manual/Automatic control.
Control Content: Digital setting with delayed quantitative control.
Actuator: Peristaltic pump or automatic control valve.

名称

技术参数

罐体

设计压力：罐体设计压力≤0.3MPa,工作压力≤0.15MPa,采用优化导流设计；内外抛光精度Ra≤0.4,罐体罐体配备：人孔，视镜，pH电极、DO电极、温度电极接口。Tanks罐盖配有火焰接种口和各类工艺接口。

搅拌

顶部机械搅拌系统；无菌密封系统；搅拌搅拌结构：方便拆卸，可调式搅拌轴及机械密封总成。Mixer搅拌桨叶：多形式组合式搅拌桨叶，1级梳齿高效消泡桨(标配)。

进气系统

进气方式：罐底深层通气。(选配气体环状分布器)进气系统除菌过滤：配备预过滤系统，精度03μm;除菌过滤系统，精度为001μm;

管路系统

管路安装符合微生物发酵要求，无死角，无物料残留。管路系统与物料接触管路阀门采用卫生级隔膜阀和内壁抛光管。

设备

控制功能

温度

pH

DO

转速

流量

压力

灭菌

补料

种子罐

测控

测控

测控

变频

自动/手动

自动/手动

自动/手动

蠕动泵/自动隔膜阀

发酵罐

测控

测控

测控

变频

自动/手动

自动/手动

自动/手动

蠕动泵/自动隔膜阀

控制参数

技术指标

温度

四位数显温度变送器，配套PT100温度电极

测量精度：0.3级(0.3%FS±0.1℃),

测量范围：0-150℃

控制方式：数字化设定，手/自动控制

执行机构：气动阀门/热水循环泵

控制内容：双向控制加热和冷却

pH

世界品牌瑞士公司pH玻璃凝胶电极

电极可高温在位灭菌，灭菌温度：100℃-130℃,

pH配套不锈钢pH电极护套；

测量范围：0-14,

精度：±0.01mm;四位数显变送器，

供电AC 220V,4-20mA

配备原装瑞士进口电极屏蔽线

DO

世界品牌瑞士公司DO膜电极，

电极可高温在位灭菌，灭菌温度：100℃-130℃,

配套不锈钢DO电极护套；

测量范围：0-100%,精度±1%;

配套四位数显变送器，供电AC 220V,4-20mA

转速

转速变频无级调速系统，功率与电机匹配

数字化设定转速数值

执行机构：变频器

灭菌

数字化设定罐内灭菌和灭菌时间，自动控制灭菌温度和降温过程。

灭菌温度范围：80℃-125℃。

灭菌灭菌过程：蒸汽通过夹套加温，对罐内预热；达到预热温度，可选择设定蒸汽直接进罐，使罐内温度达到灭菌温度；

开始计时时并自动控制灭菌温度；

达到灭菌时间后自动降温至设定发酵温度。

压力

数字化设定罐内压力，4-20mA信号线性调节

罐内压力

流量（选配）

数字化设定进气流量，比例调节阀自动控制

消泡

控制方式：手/自动控制

控制内容：数字化设定延时定量控制

执行机构：具有泡沫报警功能

补料

控制方式：手/自动控制

控制内容：数字化设定延时定量控制

执行机构：蠕动泵控制或自控阀控制