PurePep Chorus多肽合成仪

UNCOMPROMISING PURITY USING A MODULAR DESIGN AND INTUITIVE SOFTWARE WORKFLOW

不妥协的纯度使用模块化设计和直观的软件工作流程

Pre-programmed standard methods – use as is, or as a template for customized methods

Import sequences – easily create syntheses from focused libraries

Multi-task – plan one synthesis while another is running

Auto-calculations – reagent preparation and usage calculators built into the software Configurable on all vessel pairs – rapid, independent induction heating and real-time UV monitoring Upgradeable throughput – from 2 to 4 to 6 reaction vessels

预先编程的标准方法——按原样使用，或者作为定制方法的模板  

导入序列-很容易从集中的库创建合成  

多任务-计划一个合成，当另一个合成正在运行  

自动计算-试剂制备和使用计算器内置到软件中可配置在所有容器对-快速，独立的感应加热和实时紫外线监测可升级的吞吐量-从2到4到6反应容器

MULTI-SCALE   PEPTIDE  SYNTHESIZER

多尺度多肽合成仪

7 EASY STEPS TO PEPTIDE SYNTHESIS USING PUREPEP CHORUS

7个简单的步骤合成肽使用PUREPEP CHORUS

Step 1: Group reaction vessels to run simultaneously or sequentially. Different groups can run unique protocols.  

步骤1:将反应容器分组，同时或顺序运行。 不同的组可以运行唯一的协议。 
 

Step 2: Input parameters including coupling methods, reagent excess, and information on resins for use in record-keeping and calculations.

步骤2:输入参数，包括偶联方法，试剂过量，以及用于记录和计算的树脂信息。

Step 3: Add peptides from the library or create new ones with the sequence editor. Select cycles for special chemistry within groups and prime-free Single-ShotTM additions for expensive reagents.  

步骤3:从库中添加多肽或用序列编辑器创建新的多肽。 选择循环组内的特殊化学和无底质的single-shotTM添加昂贵的试剂。
  

Step 4: Choose to use default bottle positions for amino acids and reagents or arrange as desired.  

步骤4:选择使用氨基酸和试剂的默认瓶位或按需要排列。

Step 5: Assign pre-programmed or custom chemistry protocols to any cycle in any group. For heated syntheses, select default temperatures for deprotections and couplings, and exceptions for any step, cycle, RV or group.

步骤5:将预编程或自定义的化学协议分配给任何小组的任何循环。 对于加热合成，为脱保护和耦合选择默认温度，并为任何步骤、周期、RV或组选择例外温度。

Step 6: Let the software calculate the weights and volumes needed for all reagents, as well as theoretical yield and estimated synthesis time.

步骤6:让软件计算出所有试剂所需的重量和体积，以及理论产率和估计合成时间。

Step 7: Start synthesis from the beginning or at any cycle and step desired. View UV and temperature data in real time while a synthesis is running, and follow countdown to completion of your peptide.

步骤7:从一开始或在任何所需的循环和步骤开始合成。 查看紫外和温度数据实时合成运行，并遵循倒计时完成您的肽。

IntellisynthTM UV Monitoring

IntellisynthTM紫外线监控

Proprietary Intellisynth real-time UV monitoring can be configured on as many reaction vessel pairs as you desire, enabling automated optimization of the deprotection reaction. Monitoring the solution during mixing allows control of both reaction times and the number of repetitions, ensuring deprotections go to completion while avoiding wasted time or reagent. 

Intellisynth实时紫外监测可以配置在任意多对反应容器上，实现去保护反应的自动化优化。 在混合过程中监控溶液可以控制反应时间和重复次数，确保去保护完成，同时避免浪费时间或试剂。  

View real-time UV data while a synthesis is running and full data from any completed synthesis.

查看实时紫外数据，而合成运行和完整的数据从任何完成的合成。

Individual graphs show reaction progress during a cycle.  Summary graphs indicate differences in times and repetitions between different cycles, providing valuable diagnostic information on synthetic trouble spots for difficult sequences.

单个图表显示了一个周期内的反应进展。 摘要图显示了不同循环之间的时间和重复的差异，为复杂序列的合成故障点提供了有价值的诊断信息。  

Induction heating

感应加热

Induction heating can be configured on as many reaction vessel pairs as you desire. It accelerates chemistry and improves purity for difficult sequences. Heating conditions in each of the six reaction vessels can be independently set to enable temperature scans for process optimization or to accommodate parallel synthesis of sequences requiring different protocols. View real-time temperature data while a synthesis is running and full profiles from any completed synthesis.

感应加热可以配置在你想要的许多反应容器对。 它加速了化学反应，提高了复杂序列的纯度。 六个反应容器中的加热条件可以独立设置，以进行温度扫描，以进行工艺优化，或适应需要不同方案的序列的并行合成。 在合成过程中查看实时温度数据，以及任何已完成合成的完整剖面。  

21 CFR part 11 compliance and IQ/OQ/PQ

PurePep software has been designed with 21 CFR part 11 compliance in mind, ensuring the instrument can be used in highly regulated cGMP laboratory environments. IQ/OQ support and PQ guidance are also available.

PurePep软件设计时考虑了21 CFR part 11的遵从性，确保仪器可以在高度规范的cGMP实验室环境中使用。 IQ/OQ支持和PQ指导也是可用的。 

Key software features include:

主要软件功能包括:

User management用户管理

Electronic signatures电子签名

Audit trail审计追踪

Easy-to-review reports for maximum traceability易于审查的报告，最大的可追溯性
  

PURITY: PurePep Pathway dedicated chemically inert pathways 

PRODUCTIVITY: flexible configuration and in-lab upgradeable 2, 4, 6 RVs 

POWER: independent, controlled induction heat and real-time UV monitoring

纯度:purepp途径专用的化学惰性途径  

生产力:灵活的配置和实验室可升级的2、4、6RVs  

电源:独立、可控的感应加热和实时紫外监测 

Purity starts with PurePep™ pathway inside with proprietary microfluidics that minimize cross-contamination, dead volumes, and reagent carryover. 

纯度从PurePep™通路内部开始，使用专有的微流体，最大限度地减少交叉污染、死体积和试剂携带。  

SYSTEM SPECIFICATIONS系统主要技术指标

Number of reaction vessels：2, 4, 6 configurable with in-lab upgrades

反应容器数量：2，4，6可通过实验室升级进行配置

Synthesis scale range：0.005 - 1.0 mmol per reaction vessel (6.0 mmol total for a 6 RV system) 

合成刻度范围：0.005 - 1.0 mmol /个反应容器(6 RV系统总浓度为6.0 mmol) 

Reaction vessel volume：Plastic, disposable - 10 mL, 45 mL；Borosilicate glass - 10 mL, 25mL, 40 mL

反应容器体积:塑料，一次性- 10ml, 45ml;硼硅酸盐玻璃- 10ml, 25mL, 40ml  

Number of solvent positions：8 solvents (1 to 4 L each standard, larger vessels available) 10mL, 25mL or 45 mL

溶剂位置的数量:8溶剂(1到4 L每标准，更大的容器可用)10mL, 25mL或45ml 

Number of amino acid positions：27 amino acid bottles or Single-Shots plus six Single-Shot only positions for specialty reagents 10 mL (for standard or Single-Shot delivery for specialty reagents), 50 mL (Single-Shot only positions), 120 mL, and 400 mL sizes.

Pre-packaged and empty bottles available

氨基酸位置的数量:27个氨基酸瓶或单针加6个单针专用试剂位置10ml(用于标准或专用试剂的单次注射)，50ml(仅用于单次注射位置)，120ml和400ml大小。可提供预包装和空瓶

Chemistries supported：Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA  

支持的化学:Fmoc, t-Boc，有机，类肽，组合，分支，PNA 

Activation：in situ and pre-activation

激活:原位和预激活

Fluid transfer method：Positive pressure with nitrogen

流体输送方法:正压氮气输送

Agitation method：Nitrogen bubbling - adjustable, and/or optional

搅拌方式:氮气鼓泡-可调，和/或可选 

Mixing method：oscillating shaker mixing

混合方法:振荡式振动筛混合

Heating method：Controlled induction heating, configurable to the number of RVs

加热方式:可控感应加热，可根据RVs数量配置

Cleavage:Automated on-board cleavage, separate fluid path and vent

解理:自动板载解理，独立的流体通道和排气口

Waste container:(1) 20 L D.O.T rated container with over-flow sensor in cap 

废物容器:(1)20升的额定运输量容器，瓶盖上有溢出传感器

Reporting:Real-time log of each instrument function, print to file or printer. Includes e-mail notifications to send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or if an error occurs.

报告:实时记录各仪表功能，打印到文件或打印机。 包括电子邮件通知，用于在指定的程序步骤、每个周期的开始或发生错误时向计算机或手机发送电子邮件。

Power:100-230VAC , 50/60Hz

电源:100-230VAC , 50/60Hz

Dimensions：87.1 cm x 72.1 cm x 72.4 cm (34.3” x 28.4” x 28.5”)

尺寸：87.1 cm x 72.1 cm x 72.4 cm (34.3” x 28.4” x 28.5”)

Weight：90.7 kg (200 lbs)

重量：90.7 kg (200 lbs)

Warranty：One year, parts and labor

保修:一年，包括配件和人工